To STW website

Project 10466: Awakening The Sleeping Lantibiotics




Projectleader: Prof. Dr. Oscar P. Kuipers


A synthetic biology approach will be used to produce more than 200 mainly silent, i.e. non-expressed in common growth media, lantibiotics from various genetic sources. To search for novel silent lantibiotic gene clusters all currently available bacterial genomes will be mined by means of state of the art bioinformatics programs including a novel bioinformatic tool called BAGEL. L. lactis will be employed that overproduces the nisin synthetase complex for lantibiotic synthesis and modification.. This has the great advantage that the need of including the corresponding biosynthetic, regulatory and immunity genes for each lantibiotic can be circumvented . After production of the novel natural lantibiotics, a thorough testing of the peptides against a library of target organisms will take place, including an initial screen for mode of action analysis. The goal is to end up with at least 6 novel candidate lantibiotics that pass all tests and from which peptides will be selected for clinical trials.

People involved

Prof. dr. O.P. Kuipers
Project function: Main applicant and project leader
Organisation: University of Groningen

Prof. dr. A.J.M. Driessen
Project function: Fellow applicant
Organisation: University of Groningen

Dr. G.N. Moll
Project function: Fellow applicant
Organisation: BioMade Technology

A.J. van Heel
Project function: PhD student
Organisation: University of Groningen

H.L. Zwart
Project function: PhD student
Organisation: University of Groningen

A. Plat
Project function: Technician
Organisation: BioMade Technology

Contact information

Prof. dr. O.P. Kuipers
Rijksuniversiteit Groningen
Faculteit Wiskunde en Natuurwetenschappen
Groningen Biomolecular Sciences and Biotechnology Institute
Postbus 14
9750 AA HAREN GN

T: 050-363 20 92
F: 050-363 23 48
E: o<dot>p<dot>kuipers<at>rug<dot>nl